The genomics revolution has enhanced the need to detect gene sequences, protein interactions and other biological reactions in drug discovery, biomedical research and clinical diagnostics. As a result, technologies have emerged allowing the simultaneous analysis of bioassays, which increases throughput and deliver results faster.
Multiplex technology allows the simultaneous qualitative or quantitative analysis of analytes using color-coded microspheres in a single tube or well.
Precise concentrations of two fluorescent dyes have been used to internally dye 100 distinctly colored bead sets to create a 100 bead array (map). Each bead can be coated with reagents specific for the bioassay, including antigens, antibodies, oligo-nucleotides, or enzyme substrates. A reporter molecule is labeled with a fluorescent dye for qualitative or quantitative analysis of the bioassay.
The advantages of multiplex technology over other bioassays are numerous: